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. 2005 Apr;17(4):1061–1072. doi: 10.1105/tpc.104.029819

Table 3.

Validation of Genes with Significant Differential Expression during Early Endosperm Formationa

GenBank Entry P Valuesb Ratioc Log U.limd Log L.lime Predicted Function RT-PCRf
AI881293 0.0015 1.63 1.281 0.135 ATPase inhibitor −
AI649910 0.0001 3.03 2.170 1.024 Aconitate hydratase +
AI665004 0.0001 0.42 −0.671 −1.839 F-box protein +
AI861494 0.0004 1.72 1.355 0.209 Galactosyltransferase +
AI438413 0.0003 1.76 1.400 0.233 Helicase +
AI629534 0.0020 0.63 −0.087 −1.233 Importin −
AI820392 0.0007 1.70 1.352 0.184 γ-Zein mRNA +
AI770450 0.0008 0.60 −0.160 −1.327 Membrane protein +
AI820386 0.0001 3.04 2.177 1.031 Metal binding protein +
AI734543 0.0001 0.55 −0.281 −1.449 GTP binding protein −
AI881878 0.0001 2.01 1.594 0.427 Receptor kinase −
AI622733 0.0003 1.75 1.379 0.233 Replication protein +
AI714918 0.0001 2.63 1.979 0.811 Ribosomal protein +
AI461564 0.0006 0.59 −0.190 −1.336 RNA binding protein +
AI820302 0.0011 1.66 1.302 0.156 sup1 +
AI677072 0.0001 0.19 −1.794 −2.962 Tumor protein +
AI461566 0.0041 0.64 −0.066 −1.234 Unknown protein −
AI619247 0.0010 0.61 −0.139 −1.307 Unknown protein +
AI665151 0.0001 2.63 1.979 0.812 Unknown protein −
AI714900 0.0001 1.62 1.109 0.290 Unknown protein +
AI715072 0.0012 1.67 1.319 0.152 Unknown protein +
AI737979 0.0040 0.64 −0.061 −1.207 Unknown protein −
AI770943 0.0030 1.57 1.221 0.075 Unknown protein +
AI833477 0.0042 0.64 −0.064 −1.210 Unknown protein +
AI834598 0.0001 0.32 −1.242 −2.061 Unknown protein +
AI855049 0.0040 1.57 1.234 0.066 Unknown protein +
AI861472 0.0040 1.54 1.197 0.051 Unknown protein +
a

Twenty-seven genes identified from the microarray data were validated with semiquantitative RT-PCR.

b

Individual P values obtained from the microarray data with the mixed linear model.

c

Ratio calculated using the mean of the adjusted signal intensity values.

d

Log value of the upper limit of the confidence interval.

e

Log value of the lower limit of the confidence interval.

f

The plus sign indicates data confirmed by semiquantitative RT-PCR (i.e., for which the microarray data and the RT-PCR indicate a change in the same direction when comparing mature unfertilized ovules and ovules 3 DAP); the minus sign indicates no significant difference detected by RT-PCR.