Figure 7.

ZsG antigen promotes cDC maturation. A. Diagram illustrating the overall question of whether ZsG acquisition is required for cDC1 and/or cDC2 activation. Data in this figure interrogate ZsG⁺ cDCs, so the ZsG^- portion is shaded. B & C. Bar graphs of percent of ZsG⁺ cDC1s (B) and cDC2s (C) that are activated (CD86^hiMHCII^hi). n=10 per group. Unpaired Welch's t-test: ∗ p < 0.05. D-E & H-I. Geometric mean fluorescent (GMF) intensity of CD86 on ZsG^- and ZsG⁺ cDC1s (D-E) and cDC2s (H-I). n = 10. Paired t-test: ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. F & J. CD86 expression as a function of ZsG expression in ZsG⁺ cDC1s (F) and ZsG⁺ cDC2s (J) after sham treatment. G & K. CD86 expression as a function of ZsG expression in ZsG⁺ cDC1s (G) and ZsG⁺ cDC2s (K) after BH. Correlations are by linear regression with displayed R² values.