Table 1.
Genes Downregulated in rpk1-2 Seedlings as Identified in Microarray Analysis
| Experiment 1a
|
Experiment 2b
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Genec | AGI Code | Ratio(±sd)d | P Value 1e | P Value 2e | Ratio(±sd)d | P Value 1e | P Value 2e | ABA Signal Transductionf | ABRE Positiong |
| Glycosyl hydrolase family 19 (chitinase) | AT2G43620 | 0.12 (0.03) | 2.9E-29 | 2.8E-26 | 0.15 (0.02) | 1.1E-26 | 1.0E-24 | ||
| 2S storage protein-like | AT5G54740 | 0.16 (0.01) | 1.9E-25 | 7.2E-24 | 0.44 (0.02) | 2.6E-10 | 1.8E-08 | ABRE, Abi3 | −64 |
| Extensin4 | AT1G76930 | 0.17 (0.02) | 2.8E-25 | 7.9E-24 | 0.27 (0.04) | 4.5E-20 | 9.2E-17 | ||
| Peroxidase, putative | AT5G64120 | 0.19 (0.02) | 2.7E-24 | 2.1E-22 | 0.10 (0.01) | 3.0E-28 | 2.5E-29 | ABRE, induction | −61 |
| Late embryogenesis abundant M17 protein | AT2G41260 | 0.22 (0.03) | 1.7E-22 | 6.1E-20 | 0.58 (0.05) | 1.6E-06 | 2.8E-04 | ||
| 12S cruciferin seed storage protein | AT4G28520 | 0.24 (0.02) | 5.2E-21 | 1.7E-19 | 0.44 (0.06) | 1.8E-05 | 8.3E-04 | ABRE, Abi3 | −108 |
| Unknown protein | AT1G14880 | 0.25 (0.14) | 5.6E-21 | 6.1E-11 | ABRE | −734 | |||
| Peroxidase, putative | AT5G39580 | 0.26 (0.04) | 1.2E-20 | 1.5E-17 | 0.14 (0.03) | 3.9E-27 | 4.7E-25 | Induction | −915 |
| Sugar transporter-like protein | AT4G36670 | 0.26 (0.01) | 1.6E-18 | 1.6E-17 | 0.34 (0.00) | 9.0E-15 | 1.0E-14 | Induction | −402 |
| pEARLI 1-like protein | AT4G12470 | 0.32 (0.01) | 3.9E-11 | 1.1E-09 | 0.56 (0.06) | 7.0E-08 | 1.5E-05 | ||
| Expressed protein | AT2G25510 | 0.32 (0.03) | 7.7E-17 | 1.3E-14 | 0.60 (0.06) | 1.9E-01 | 6.1E-01 | −935 | |
| Putative RLK | AT2G19190 | 0.33 (0.03) | 1.7E-14 | 1.9E-12 | 0.10 (0.01) | 1.4E-29 | 3.3E-28 | ||
| FAD-linked oxidoreductase family | AT1G30720 | 0.34 (0.01) | 5.4E-15 | 1.4E-14 | 0.19 (0.01) | 2.3E-23 | 6.6E-23 | Induction | |
| MMK2 | AT1G59580 | 0.34 (0.02) | 3.3E-13 | 2.9E-11 | 0.18 (0.05) | 7.6E-25 | 3.8E-20 | −904 | |
| Legumin-like protein | AT5G44120 | 0.36 (0.08) | 2.6E-15 | 1.8E-10 | 0.8 (0.03) | 2.3E-02 | 9.2E-02 | −588 | |
| Receptor protein kinase, putative | AT1G51800 | 0.36 (0.04) | 1.2E-13 | 1.2E-06 | 0.21 (0.01) | 1.7E-22 | 3.0E-21 | ||
| Oleosin | AT4G25140 | 0.37 (0.09) | 3.4E-10 | 3.4E-10 | ABRE, Abi3 | −185 | |||
| Light-repressible receptor protein kinase, putative | AT1G51850 | 0.41 (0.04) | 5.5E-12 | 6.4E-09 | 1.28 (0.04) | 2. 8E-19 | 3.7E-16 | ||
| Unknown protein | AT2g05580 | 0.41 (0.12) | 9.0E-11 | 5.4E-13 | |||||
| FAD-linked oxidoreductase family | AT1G30730 | 0.42 (0.01) | 1.3E-08 | 7.2E-08 | 1.21 (0.04) | 2. 0E-22 | 4.8E-19 | Induction | |
| Integral membrane family protein | AT4G15620 | 0.42 (0.03) | 9.9E-09 | 7.9E-08 | −91 | ||||
| UTP-glucose glucosyltransferase-like protein | AT3G50740 | 0.42 (0.06) | 4.5E-13 | 1.2E-09 | 0.65 (0.03) | 2.6E-05 | 1.3E-03 | Induction | |
| Expressed protein | AT3G22640 | 0.43 (0.00) | 1.1E-10 | 1.7E-10 | 0.72 (0.07) | 1.1E-03 | 1.7E-02 | −411 | |
| Putative protease inhibitor | AT2G38870 | 0.43 (0.02) | 2.6E-11 | 3.8E-10 | 0.21 (0.03) | 3.0E-23 | 4.3E-21 | −601 | |
| 2-Oxoglutarate-dependent dioxygenase family | AT2G36690 | 0.44 (0.24) | 5.4E-13 | 8.8E-04 | 0.17 (0.00) | 1.6E-24 | 2.5E-24 | −772 | |
| Nucleoid DNA binding protein cnd41-like protein | AT5G10760 | 0.44 (0.05) | 1.2E-07 | 1.4E-07 | 0.96 (0.05) | 9.7E-01 | 9.9E-01 | −238 | |
| Expressed protein | AT1G61380 | 0.45 (0.04) | 1.9E-05 | 2.6E-05 | 0.41 (0.08) | 2.0E-13 | 4.3E-09 | Induction | |
| Expressed protein | AT1G52200 | 0.45 (0.09) | 1.1E-12 | 1.0E-07 | 0.39 (0.06) | 2.0E-14 | 7.9E-11 | ||
| Peroxidase, putative | AT5G05340 | 0.45 (0.12) | 9.8E-10 | 4.4E-05 | Induction | −922 | |||
| Type 2 peroxiredoxin, putative | AT1G65970 | 0.46 (0.02) | 3.0E-10 | 5.8E-09 | 0.27 (0.05) | 1.6E-20 | 1.8E-16 | ABRE, Abi3 | |
| Glucuronosyl transferase-like protein | AT3G46690 | 0.47 (0.06) | 1.0E-09 | 8.9E-06 | 0.30 (0.05) | 1.8E-18 | 3.9E-15 | −89 | |
| Glutathione transferase, putative | AT1G69930 | 0.47 (0.12) | 2.4E-09 | 4.8E-06 | Induction | −480 | |||
| Glycosyltransferase family | AT3G46700 | 0.48 (0.00) | 2.2E-08 | 7.1E-07 | 0.34 (0.01) | 6.4E-12 | 1.1E-10 | ||
| Similar to Mlo proteins from Hordeum vulgare | AT2G39200 | 0.48 (0.13) | 1.1E-11 | 1.1E-05 | 0.25 (0.06) | 1.0E-21 | 3.5E-17 | ||
| Peroxidase, putative | AT4G36430 | 0.49 (0.15) | 1.4E-10 | 1.2E-04 | 0.44 (0.03) | 4.4E-11 | 1.4E-09 | ||
| FAD-linked oxidoreductase family | AT1G26420 | 0.49 (0.02) | 1.8E-08 | 3.7E-08 | 0.20 (0.08) | 2.1E-22 | 9.9E-15 | ||
| 1-Aminocyclopropane-1-carboxylate synthase-like protein | AT4G26200 | 0.49 (0.17) | 5.2E-12 | 1.7E-04 | 0.33 (0.08) | 1.6E-17 | 7.1E-12 | ||
| Protein kinase-like protein | AT4G11890 | 0.49 (0.00) | 5.9E-07 | 7.1E-07 | Induction | −550 | |||
| AP2 domain protein RAP2.3 | AT3G16770 | 0.49 (0.02) | 5.9E-09 | 3.7E-08 | 0.59 (0.00) | 7.0E-06 | 2.0E-05 | ||
a
Experiment 1: downregulated genes in ABA-treated rpk1 young plants (ABA-treated rpk1-2/ABA-treated Ws).
b
Experiment 2: downregulated genes in untreated rpk1-2 young plants (untreated rpk1-2/untreated Ws).
c
Genes with ratio (ABA-treated rpk1-2/ABA-treated Ws) of <0.5 are listed.
d
Values represent the means of two replicates. sd, standard deviations of two replicates.
e
P values of two replicates. P values < 0.0005 were studied.
f
ABA responses were detected in other experiments (Kroj et al., 2003) or by microarray analysis (K. Maruyama, unpublished data).
g
ABA response cis-sequence; ABREs were detected in the promoter.