Table 2.
Genes Downregulated in RL-BK Overexpressing Plants as Identified by Microarray Analysis
| Experiment 1 (Transgenic Line L1)a
|
Experiment 2 (Transgenic Line L3)b
|
||||||
|---|---|---|---|---|---|---|---|
| Genec | AGI Code | Ratio (±sd)d | P Value 1e | P Value 2e | Ratio (±sd)d | P Value 1e | P Value 2e |
| Unknown protein | AT5G64770 | 0.12 (0.04) | 4.7E-26 | 1.3E-23 | 0.19 (0.00) | 1.2E-21 | 1.5E-21 |
| Auxin-induced protein-like | AT5G18020 | 0.16 (0.02) | 2.5E-22 | 2.7E-21 | 0.22 (0.04) | 1.3E-20 | 5.3E-17 |
| Thionin, putative | AT1G66100 | 0.17 (0.00) | 1.5E-21 | 1.2E-19 | 0.24 (0.00) | 8.0E-18 | 3.7E-17 |
| GAST1-like protein | AT1G74670 | 0.17 (0.02) | 3.0E-25 | 2.5E-23 | 0.18 (0.02) | 2.0E-24 | 5.1E-23 |
| Fructose-bisphosphate aldolase-like protein | AT4G26530 | 0.18 (0.04) | 2.5E-25 | 5.8E-22 | 0.23 (0.01) | 1.3E-20 | 4.5E-20 |
| Expansin, putative | AT2G40610 | 0.19 (0.03) | 2.7E-24 | 1.1E-21 | 0.16 (0.01) | 3.7E-25 | 3.3E-24 |
| Auxin-induced protein, putative | AT1G29500 | 0.21 (0.07) | 1.3E-23 | 1.6E-17 | 0.27 (0.05) | 1.2E-18 | 1.4E-15 |
| Expressed protein | AT4G18970 | 0.25 (0.03) | 4.9E-21 | 9.9E-19 | 0.25 (0.02) | 1.3E-20 | 5.0E-19 |
| Early auxin-induced protein, IAA19 | AT3G15540 | 0.26 (0.12) | 1.3E-17 | 1.1E-11 | 0.41 (0.03) | 7.0E-11 | 8.5E-09 |
| Auxin-induced protein-like | AT5G18030 | 0.29 (0.03) | 1.1E-17 | 1.7E-15 | 0.29 (0.04) | 4.7E-18 | 1.4E-14 |
| Expressed protein | AT5G52900 | 0.31 (0.05) | 5.0E-18 | 1.4E-14 | 0.39 (0.03) | 2.7E-13 | 1.3E-11 |
| Expressed protein | AT1G32080 | 0.31 (0.00) | 4.2E-16 | 8.3E-16 | 0.37 (0.00) | 2.0E-13 | 2.7E-13 |
| Auxin-induced protein (SAUR), putative | AT5G18010 | 0.32 (0.04) | 1.1E-15 | 5.5E-13 | 0.38 (0.02) | 1.9E-12 | 3.4E-11 |
| myb-related transcription factor mixta, putative | AT1G18710 | 0.32 (0.02) | 4.6E-15 | 5.9E-13 | 0.44 (0.08) | 3.1E-11 | 1.6E-07 |
| Putative auxin-induced protein AUX2-11 | AT1G04240 | 0.33 (0.03) | 3.8E-16 | 9.4E-14 | 0.42 (0.02) | 1.8E-11 | 2.4E-10 |
| Putative protein | AT5G11420 | 0.33 (0.05) | 5.5E-17 | 9.4E-14 | 0.34 (0.04) | 3.7E-16 | 2.1E-13 |
| Auxin-induced protein-like | AT5G18060 | 0.33 (0.06) | 3.0E-16 | 2.2E-13 | 0.37 (0.03) | 8.2E-15 | 3.1E-14 |
| Auxin-induced protein-like | AT5G18080 | 0.33 (0.15) | 1.1E-19 | 2.2E-09 | 0.38 (0.10) | 9.7E-15 | 3.0E-09 |
| Methyladenine glycosylase family protein | AT5G44680 | 0.34 (0.16) | 5.8E-17 | 4.5E-13 | 0.37 (0.03) | 3.1E-14 | 2.7E-12 |
a
Experiment 1: downregulated genes in untreated RL-BK line L1 (L1/control).
b
Experiment 2: downregulated genes in untreated RL-BK line L3 (L3/control).
c
Genes with ratio (untreated RL-BK transgenic plants/untreated VC plants) of <0.35 are listed.
d
Values represent the means of two replicates. sd, standard deviations of two replicates.
e
P values of two replicates. P values < 0.005 were studied.