Table 3.
Genes Upregulated in RL-BK Overexpressing Plants as Identified by Microarray Analysis
| Experiment 1(Transgenic Line L1)a
|
Experiment 2(Transgenic Line L3)b
|
|||||||
|---|---|---|---|---|---|---|---|---|
| Genec | AGI Code | Ratio (±sd)d | P Value 1e | P Value 2e | Ratio (±sd)d | P Value 1e | P Value 2e | ABA Responsef |
| BR-6-oxidase (BR6ox2) | AT3G30180 | 9.0 (0.04) | 6.3E-28 | 7.5E-28 | 8.0 (1.13) | 7.6E-28 | 4.3E-26 | BR feedbackg |
| Xyloglucan endotransglycosylase (XTR7) | AT4G14130 | 6.4 (0.37) | 1.0E-25 | 6.8E-25 | 5.1 (0.03) | 7.5E-23 | 9.2E-23 | Induction |
| Steroyl acyl carrier protein desaturase | AT1G43800 | 5.8 (2.34) | 7.1E-22 | 4.1E-17 | 2.6 (0.20) | 7.3E-11 | 2.9E-10 | |
| Oleosin | AT4G251 | 4.7 (0.45) | 1.1E-22 | 6.3E-21 | 4.2 (0.15) | 2.0E-20 | 1.2E-19 | Response |
| Expressed protein | AT1G75750 | 4.7 (1.45) | 3.2E-24 | 1.9E-18 | 3.7 (1.00) | 5.5E-21 | 4.5E-15 | |
| Legumin-like protein | AT5G44120 | 4.3 (0.09) | 5.3E-21 | 1.3E-20 | 3.4 (0.07) | 2.1E-17 | 6.1E-17 | Response |
| 12S cruciferin seed storage protein | AT4G28520 | 4.2 (0.19) | 7.1E-21 | 5.8E-20 | 3.7 (0.18) | 5.0E-19 | 5.1E-18 | Response |
| Expressed protein | AT2G19800 | 4.8 (0.89) | 1.3E-20 | 9.3E-16 | 2.8 (0.51) | 1.3E-15 | 3.3E-10 | Guard cellh |
| 2S storage protein-like | AT5G54740 | 3.8 (0.08) | 4.6E-19 | 1.3E-18 | 3.2 (0.00) | 1.9E-16 | 1.9E-16 | Response |
| Unknown protein | AT2G05580 | 3.7 (1.05) | 6.7E-20 | 7.5E-14 | 2.4 (0.07) | 9.8E-11 | 3.2E-09 | |
| Jasmonate-inducible protein, putative | AT1G52100 | 3.7 (0.48) | 9.9E-20 | 6.6E-17 | 3.1 (0.17) | 7.6E-16 | 1.3E-14 | |
| Expressed protein | AT3G02480 | 3.7 (0.03) | 1.0E-18 | 1.5E-18 | 2.2 (0.09) | 1.7E-10 | 1.7E-09 | Induction |
| Oleosin | AT5G40420 | 3.4 (0.34) | 5.3E-18 | 4.7E-16 | 2.8 (0.54) | 9.2E-16 | 3.1E-11 | Response |
| Embryo-specific protein 1 (ATS1) | AT4G26740 | 3.4 (0.88) | 2.4E-19 | 8.4E-14 | 2.8 (0.35) | 4.5E-15 | 7.7E-12 | |
| Putative protein | AT3G55240 | 3.4 (0.35) | 2.9E-18 | 5.9E-16 | 2.3 (0.07) | 4.3E-11 | 2.5E-10 | |
| Expressed protein | AT2G39030 | 3.4 (0.52) | 3.3E-18 | 2.5E-14 | 2.6 (0.27) | 2.4E-13 | 1.2E-10 | Induction |
| Embryo-specific protein 1 (ATS1) | AT5G55240 | 3.3 (0.03) | 4.0E-16 | 7.3E-16 | 2.9 (0.13) | 6.6E-14 | 4.7E-13 | |
| Plant defensin protein, putative (PDF1.4) | AT1G19610 | 3.3 (0.31) | 1.0E-17 | 1.4E-15 | 2.2 (0.29) | 2.3E-11 | 3.4E-08 | |
| Glycosyl hydrolase family 18 | AT4G19810 | 3.2 (0.65) | 6.5E-18 | 1.9E-13 | 2.8 (0.63) | 4.4E-16 | 1.0E-10 | Induction |
| Glycosyl hydrolase family 1 | AT3G21370 | 3.2 (0.13) | 1.1E-16 | 8.6E-16 | 3.0 (0.15) | 7.0E-16 | 9.7E-15 | |
| Late embryogenesis abundant protein LEA-like | AT5G06760 | 3.2 (1.12) | 4.3E-19 | 3.0E-11 | 1.9 (0.47) | 2.8E-09 | 4.7E-04 | Induction |
| Similar to chloroplast nucleoid DNA binding protein-like | AT4G16563 | 3.2 (0.69) | 2.3E-17 | 2.2E-12 | 3.1 (0.35) | 1.6E-15 | 2.6E-13 | |
| Putative seed storage protein (vicilin-like) | AT2G28490 | 3.1 (0.35) | 3.2E-17 | 1.2E-14 | 2.6 (0.11) | 7.1E-15 | 6.4E-11 | |
| Expressed protein | AT1G72450 | 3.1 (0.20) | 1.6E-16 | 5.3E-15 | 3.2 (0.13) | 5.0E-17 | 3.7E-15 | |
| Hypothetical protein | AT1G17710 | 3.1 (0.37) | 5.1E-17 | 2.5E-14 | 2.4 (0.29) | 4.3E-12 | 5.2E-11 | |
| Putative protein | AT5G26260 | 3.1 (0.21) | 1.9E-16 | 6.6E-15 | 2.1 (0.54) | 1.0E-09 | 2.8E-08 | |
| Hevein-like protein precursor (PR-4) | AT3G04720 | 3.1 (0.36) | 6.4E-17 | 3.8E-14 | 2.2 (0.30) | 9.5E-12 | 1.4E-08 | |
a
Experiment 1: upregulated genes in untreated RL-BK line L1 (L1/control).
b
Experiment 2: upregulated genes in untreated RL-BK line L3 (L3/control).
c
Genes with ratio (untreated RL-BK transgenic plants/untreated VC plants) of >3 are listed.
d
Values represent the means of two replicates. sd, standard deviations of two replicates.
e
P values of two replicates. P values < 0.005 were studied.
f
ABA responses are described in Table 1.
g
h