Table 2.
Cell Wall Composition of Wild-Type and pnt1 Embryosa
| Cell Wall Component | Wild Type | pnt1 | ||
|---|---|---|---|---|
| Celluloseb | 196 ± 17 | 117 ± 11 | ||
| Pectinc | 41 ± 3 | 93 ± 4 | ||
| Neutral sugarsd | ||||
| Rhamnose | 35 ± 3 | (7%) | 62 ± 2 | (8%) |
| Fucose | 18 ± 2 | (4%) | 20 ± 1 | (3%) |
| Arabinose | 248 ± 29 | (51%) | 416 ± 17 | (53%) |
| Xylose | 104 ± 13 | (22%) | 139 ± 4 | (18%) |
| Mannose | 18 ± 5 | (4%) | 16 ± 2 | (2%) |
| Galactose | 60 ± 6 | (12%) | 129 ± 10 | (16%) |
| Total nmol neutral sugars | 483 | 782 | ||
| Average dry weight per embryo | 4.8 μg | 3 μg |
a
Expressed as nmol sugar/mg dry weight of embryo. Value is the average of at least three measurements, with standard deviation.
b
Cellulose measured as acetic/nitric acid–insoluble glucose.
c
Pectin measured as total uronic acid after trifluoroacetic acid hydrolysis.
d
Amount of each neutral sugar as a percentage of the total neutral sugars is listed in parentheses.