Skip to main content
. 2005 Apr;17(4):1154–1166. doi: 10.1105/tpc.104.029975

Figure 6.

Figure 6.

Protein Gel Blot Analysis of GFP Protein Fusions.

Protein gel blot analysis of proteins isolated from plants carrying GFP-tagged ACR4 derivatives using an anti-GFP polyclonal antibody. Lanes 1 to 8 were immunoprecipitated with anti-GFP polyclonal antibody, and lanes 9 to 13 show total extracts. Lanes are as follows: 1, Columbia-0 (untransformed); 2, ACR4:GFP; 3, ΔREPEAT:GFP; 4, ΔTM/KIN/C-TER:GFP; 5, GFP:ACR4; 6, GFP:ACR4:GFP; 7, ΔC-TER:GFP; 8, KIN-NULL:GFP; 9, ΔREPEAT:GFP; 10, ΔTNFR:GFP; 11, ΔTM/KIN/C-TER:GFP; 12, ΔREPEAT:GFP; 13, ACR4C180-Y:GFP. The asterisk indicates the immunoglobulins remaining after elution of the immunoprecipitation. Protein loading controls (from a stained gel with identical aliquots of protein samples) are shown below lanes 9 to 13.