Table 1.
Primer Pair Combinations Used for Construction of Deletion Constructs
| Construct | N-Terminal End Amplified with: | C-Terminal End Amplified with: |
|---|---|---|
| pΔ39aa | CR5/CREC5 (see text) | CREC3 (see text)/CR3 |
| pΔ39aa:GFP | CR5/CREC5 | CREC3/GFP3STOP |
| pΔTNFR | CR5/CRTN5 (5′-AGATCTATACAGAGTCCTGGTG-3′) | CRTN3 (5′-GGATCCCAGTGGAGGCAAGGAAAAAG-3′)/CR3 |
| pΔTNFR:GFP | CR5/CRTN5 | CRTN3/GFP3STOP |
| pΔTM/K/C-ter | CR5/CRNM5 (5′-GGATCCTTTTTCCTTGCCTCCACTGG-3′) | – |
| pΔTM/K/C-ter:GFP | CR5/CRNM5 | CRCT3 (5′-AGATCTAGAATGAGTAAAGGAGAAGAAC-3′)/GFP3STOP |
| pΔC-ter | CR5/CRCTstop (5′-GAGCTCTATAGCTGTGCAAGCGCTCG-3′) | – |
| pΔC-ter:GFP | CR5/CRCT5 (5′-GGATCCCATTAGCTGTGCAAGC-3′) | CRCT3/GFP3STOP |