Figure 7.

Resistance to A. brassicicola by HDA19 Overexpression.

Graphical representation of disease symptoms in wild-type, 35S:HDA19 (G11-1 and G11-2), and HDA19-RNAi (RNAi-1 and RNAi-2) transgenic lines. Disease severity index was calculated 48 and 72 h after inoculation with A. brassicicola. Disease severity index was calculated based on the degree of symptom severity as measured by leaf necrosis area: 0, no symptom; 1, 1 to 5% necrosis areas; 2, 5 to 10% necrosis areas; 3, 10 to 25% necrosis areas; 4, >25% necrosis areas. The leaf necrosis severity ratings were summed for 60 to 80 leaves of 15 to 25 plants per genotype and divided by the number of leaves rated times the maximum possible rating to given the final disease severity index. Asterisks mark values that are significantly different from the wild type (χ² test, P < 0.05). The experiment was repeated three times with similar results.