Figure 2.
Relative Fluorescence Yield of Leaves from Arabidopsis Wild Type and npq Mutants.
Fluorescence amplitude was measured at Fm on intact leaves (A) and on leaves infiltrated (B) with either 5 mM Hepes, pH 7.0, (light gray) or 50 μM nigericin (dark gray). We used a video imaging apparatus for Fm determination (see Methods) and normalized Fm to the chlorophyll content per leaf surface. Values are the average of measurements performed on four leaves. These results have been confirmed using a PAM fluorimeter and leaf discs. Data sets with a significance level of P < 0.05 according to Student's t test are marked with an asterisk. a.u., arbitrary units.