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Flatbed IEF Fractionation.
Profiles obtained after IEF of monomeric Lhcb proteins (A) isolated from npq2 (top) and wild-type (bottom) thylakoids by sucrose gradient fractionation, monomeric Lhcb complexes (B) isolated from light-treated wild-type plants (WT-S), whose thylakoids were isolated in acidic buffer to maintain deepoxidation level, and recombinant Lhcb5 proteins (C) reconstituted in vitro with either zeaxanthin (top, CP26-Z) or violaxanthin (bottom, CP26-V).
