Figure 5.

Plant Infection and Appressorial Penetration Assays with Transformants Expressing the Dominant Active MST7 Allele.

(A) Rice leaves sprayed with conidia from ZH32C (mst7 + MST7), yk86C (mst11 + MST11), DA5 (mst11 + MST7^{S212D T216E}), DA3-4 (mst7 + MST7^{S212D T216E}), and DAC5 (mst11 + MST7).

(B) Penetration assays with onion epidermi examined under DIC (left panels) and epifluorescence (right panels) microscopy. Appressoria formed by ZH32C and yk86C penetrated and differentiated infectious hyphae at 48 h. DA5 and DA3-4 failed to penetrate onion epidermal cells but elicited autofluorescence and papilla formation in underlying plant cells. A, appressorium; C, conidium; IF, infectious hypha.