Figure 7.

Functional Characterization of the SAM and RA Domains of MST11.

(A) Amino acid residues containing the SAM and RA domains were deleted in the ΔSAM construct pYK39 and the ΔRA construct pYK45. Both pYK39 and pYK45 had the same native MST11 promoter and terminator sequences as the MST11 complementation vector pYK21.

(B) Rice leaves sprayed with conidia from transformants of yk86 expressing pYK21 (yk86C), pYK39 (MDS5), or pYK45 (MDR2).

(C) Appressorium formation assays on plastic cover slips (top panels) and penetration assays on epidermal cells of rice leaf sheaths (bottom panels) with yk86C, MDS5, and MDR2. Transformants yk86C and MDR2 but not MDS4 formed appressoria and penetrated rice leaf sheath cells. A, appressorium; C, conidium; IF, infectious hypha.