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. 2005 Apr;17(4):1317–1329. doi: 10.1105/tpc.104.029116

Figure 9.

Figure 9.

Sensitivity of the mst11 osm1 Double Mutant to Osmotic Stresses.

(A) Colony growth on 5× YEG or 5× YEG + 1 M sorbitol plates. Strains 1 to 4 were the wild-type strain 70-15, the mst11 deletion mutant yk86, the osm1 mutant JH73, and the mst11 osm1 double mutant DP5, respectively. Photos were taken after incubation at 25°C for 5 d.

(B) Colonies of JH73 (osm1) and DP5 (mst11 osm1) grew on 5× YEG + 1 M sorbitol plates for 2 weeks. No significant growth was observed in DP5.

(C) Conidia of 70-15 and the osm1 and mst11 osm1 double mutants were incubated on plastic cover slips for 24 h in DDW (top panels) or in 1 M sorbitol (bottom panels).