FIG. 2.

Expression of the GM-CSFr/PRLr chimeric receptors at the RNA level. As shown in panels A and B, Northern blot analysis of total cellular RNA from Ba/F3 cells transfected with GM-CSFr/PRLr chimeras. Ten micrograms of total RNA per lane was hybridized with the extracellular domains of the α or β subunit of hGM-CSFr as probes (panels A and B, respectively). Transfectants are indicated on top of each lane. The sizes of different chimeric transcripts in kilobases are indicated on the sides; the relative locations of the 18S and 28S bands are indicated between panels A and B. This blot was representative of several blots; not pictured here is the cotransfectant α300/βI, which demonstrated similar levels of transcript.