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DNA fragmentation and AIF translocation. (A) Pneumococci induced DNA fragmentation into large fragments of 50 kbp starting 4 hours after challenge of BMECs. M, marker. (B) In contrast, PCW stimulation was followed by DNA fragmentation into approximately 180-bp pieces. (C) Exposure of BMECs to pneumococci (107 CFU/ml) resulted in a loss of mitochondrial (MIT) AIF over time and a shift into the cytosol (CYT) after 6 hours.
