Fig. 5.

Ca_v1.2 and Ca_v3.2 are differentially expressed in A53T striatal astrocytes. a–d Representative confocal images of striatal sections co-stained with antibodies against Ca_v1.2 (a), Ca_v3.1 (b), Ca_v3.2 (c), and Ca_v3.3 (d) and the astrocyte marker, GFAP. DAPI (blue) was used for nuclei staining. Images on the right represent magnification of the boxed area. Scale bar: 50 μm (10 μm for the magnification images). e–h Quantification of mean fluorescence intensity of Ca_v VGCCs in individual GFAP⁺ astrocytes following 3D reconstruction in three independent pairs (1, 2, 3) of WT and A53T Tg mice; (e) for Ca_v1.2, ^#P < 0.0001, n ≥ 68 astrocytes for each genotype, f for Ca_v3.1, P = 0.3298, 0.1878, 0.0950 for pairs 1, 2, 3 respectively and n ≥ 44 astrocytes for each genotype, g for Ca_v3.2, ^#P < 0.0001, n ≥ 129 astrocytes, and h for Ca_v3.3, P = 0.2434, 0.2479, 0.0955, n ≥ 16 for each genotype. i Pre-averaged values of Ca_v mean fluorescence intensity relative to WT for 3 WT and A53T animal pairs. Data are presented as means ± SEM. All statistics by unpaired Student’s t test