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. 2024 Feb 21;22:142. doi: 10.1186/s12964-024-01524-x

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Calcium deficiency induces peroxisomal protein degradation in a time-dependent manner. A Immunoblot analysis of AML12 cells in calcium-deficient medium for indicated durations. Whole cell lysates were reacted with anti-catalase, anti-Pex14, anti-PMP70, anti-Acox1, anti-Pex3, anti-Pex1, anti-Pex19, anti-Pex11β, and anti-β-actin for protein expression analyses. B AML12 cells in calcium-deficient medium for indicated durations were fixed and immunostained with anti-PMP70 (red), shown as representative fluorescence image. Scale bar represents 25 µm. C Quantification of PMP70 puncta represents the number of peroxisomes per cell. Data are expressed as means ± S.D. (n = 3, independent experiments, 30 cells were analyzed in each experiment), * p < 0.05. D Immunoblot analysis of AML12 cells in calcium-deficient medium for indicated durations. Whole cell lysates were reacted with anti-VDAC, anti-Calnexin, and anti- β-actin