miR-29a expression is downregulated in GSCs, GBM cells, and human tumors. a Quantitative RT-PCR of miR-29a in GSCs and GBM cells showing lower expression levels in GSCs isolated from GBM tumors by CD133+ as compared to normal neural stem cells (NSCs) (CD133+ ) isolated from normal brain. b Quantitative RT-PCR of miR-29a in GBM surgical specimens (G) showing lower levels of miR-29a than in normal brain (N). c Quantitative RT-PCR of miR-29a in GBM cell lines (U251, U87, U373, U1242 and T98G) and GSCs (GSC-11, GSC-20, GSC-28, GSC-267, GSC-295 and GSC-627) showing lower levels of expression as compared to normal human astrocytes. *P < 0.05