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. Author manuscript; available in PMC: 2024 Feb 21.
Published in final edited form as: Cancer Discov. 2023 Aug 4;13(8):1844–1861. doi: 10.1158/2159-8290.CD-22-1200

Figure 7. Enforced expression of Nkx6-2 in a cell line derived from Kras;Gnas mice.

Figure 7.

A) Heatmap showing normalized expression values of genes differentially expressed in the Kras;GnasNkx6-2+ versus control cell line that were also found to be differentially expressed in Nkx6-2 + vs Nkx6-2 – spots in the ST dataset from Kras;Gnas mice. Expression data is provided for three RNA-seq replicates. Note that genes with average raw counts < 10 from were not included in the heatmap. B) Enrichment of the gastric isthmus and pit cell gene sets in the Nkx6-2 overexpressing cell line (Kras;GnasNkx6-2+). C) Cell proliferation assay using the Kras;GnasNkx6-2+ or control cells. * P < 0.05. D) Western blot of cell lysates obtained from the control and Kras;GnasNkx6-2+ cells, stained with NKX6-2, E-Cadherin, Vimentin, ZEB1, ZEB2 and β-actin antibodies. E) Representative H&E images of the tumors derived from the control or Kras;GnasNkx6-2+ cells at day 14 after orthotopic injection into pancreas of littermate (Kras;Gnas) or immunodeficient nude mice (NuJ). Images were captured under 10X or 40X (inset) magnification.