FIG. 6.

Hip involvement in PR assembly. (A) RL that was mock depleted or immunodepleted of endogenous Hip was used for assembly of PR complexes in vitro. The resulting PR complexes (PR lanes) and the total RL used for assembly reactions (RL lanes) were immunostained for the presence of Hip. (B) Purified recombinant Hip forms were added to RL at a 10-fold molar excess over endogenous Hip levels. The RL mixtures were used for PR assembly reactions, and the resulting PR complexes were separated by SDS-PAGE and visualized by Coomassie blue staining. The sample in the first lane contained no added protein, and the sample in the final lane (control) lacked PR in the assembly reaction mixture. Proteins associated with PR (recombinant chicken PR-A [cPRA]), along with the PR22 heavy chains (HC), are indicated on the left.