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Dose dependence and specificity of PR assembly inhibition. (A) PR complexes were assembled in RL containing the concentrations of added APAV2 or N-303 indicated above the lanes. Protein components on the resulting resin complexes were separated by SDS-PAGE and Coomassie stained. (B) Similar PR reconstitutions were performed with Hip mutants replaced by the concentrations of the Hsp70 substrate α-casein or RCMLA indicated above the lanes.
