Fig 1.

Transformation and maintenance of pCasPA into Burkholderia strains. (A) Plasmid pCasPA was mobilized into Burkholderia by triparental mating and the frequencies of conjugation are presented. After preparing L-arabinose-induced electrocompetent cells from single colonies harboring pCasPA grown in the presence of tetracycline, the percentage of cells that kept pCasPA is shown in the last column. (B) Growth curves of B. multivorans ATCC 17616/pCasPA in Lennox broth medium supplemented with tetracycline 200 µg/mL (n = 12) show that the expression of the plasmid-encoded Cas9 induced with L-arabinose up to 2% does not affect growth. The standard deviation of each assay is below 5%.