Fig 2.

Deletion of rpfR coding region from B. multivorans P0213-1 genome and mutant genotype confirmation. Electrophoretic separation of the PCR products amplified using two different primer sets from the wild-type strain P0213-1 and two colonies with the deletion of the rpfR coding sequences (locus tag: FEP09_03956 and FEP09_03957) before (A) and after (B) plasmid curing. (C) Confirmation of the genotype of the ΔrpfR deletion mutant derived from colony 7 by Sanger sequencing. The deleted region is underlined.