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Preparation of recombinant PcHGD1 and PcHGD2 and oxygen consumption during HGD reactions with HGA as a substrate. (A) SDS-PAGE analysis of purified PcHGD1 (lane 1) and PcHGD2 (lane 2). (B) Oxygen consumption during HGD reactions with HGA. Consumption of oxygen was monitored using a Clark O2 electrode. The arrow indicates the addition of a substrate to the reaction mixture.
