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Optimal temperature and pH of PcHGD1 and PcHGD2. (A and B) Optimal temperatures for PcHGD1 and PcHGD2 determined using MHQ as the substrate. Enzyme reactions proceeded at temperatures ranging from 20°C to 70°C. (C and D) Optimal pH of PcHGD1 and PcHGD2. Enzyme reactions proceeded over a pH range of 5.5–8.0: in 50 mM MES (pH 5.0–6.5; ●), 50 mM MOPS (pH 6.5–7.0; ■), and 50 mM HEPES (pH 7.0–8.0; ▲). Data are presented as mean ± standard deviation of four independent experiments.
