TABLE 1.
Comparison of phenotypic characteristics between strain NLcol2T and four other isolated strains in the Kiritimatiellota phyluma
| Strains | P. agarivorans NLcol2T | P. desulfatans F1T a) | P. sulfatireligans F21T a) | K. glycovorans L21-Fru-ABT b) | T. aerotolerans S-5007T c) |
|---|---|---|---|---|---|
| Isolation source | Microbial mat on marine sediment | Anoxic marine sediment | Anoxic marine sediment | Hypersaline microbial mat | Marine sediment |
| Cell shape | Spherical | Spherical | Spherical | Spherical | Spherical |
| Cell size (μm) | 1.0 | 0.5–1.2 | 0.5–1.0 | 1.0–2.0 | 0.5–0.8 |
| Motility | - | - | - | - | - |
| Genome size (Mbp) | 4.44 | 8.66 | 7.40 | 2.95 | 3.88 |
| DNA G + C content (mol%) | 52.4 | 56.0 | 54.6 | 63.3 | 53.1 |
| Major cellular fatty acids (>10% of total) | C18:0, i-C12:0, i-C18:0 | C18:0, i-C12:0, i-C14:0 | C18:0, i-C12:0, i-C18:0 | i-C14:0, C18:0 | C18 : 0, i-C12 : 0, i-C18 : 0, i-C16 : 0 |
| Catalase activity | - | - | - | - | Weak |
| Oxidase activity | - | - | + | - | - |
| Growth temperature (°C) | |||||
| Range | 10–37 | 10–30 | 0–25 | 20–40 | 15–45 |
| Optimum | 31 | 25 | 25 | 28 | 33–35 |
| Growth salinity (g/L NaCl) | |||||
| Range | 10–60 | 10–31 | 10–50 | 20–180 | 5–80 |
| Optimum | 25–30 | 23 | 23 | 60–70 | 30–40 |
| Growth pH | |||||
| Range | 6.0–9.0 | 6.5–8.5 | 6.0–8.5 | 6.5–8.0 | 6.0–8.5 |
| Optimum | 8.0 | 7.5 | 7.5 | 7.5 | 7.0–7.5 |
| Substrate utilization | |||||
| Glucose | + | + | + | + | + |
| Galactose | + | + | + | +/- | + |
| Fructose | + | + | + | - | + |
| Fucose | - | + | + | - | NA |
| Rhamnose | - | + | + | +/- | + |
| Mannose | + | - | + | + | - |
| Mannitol | + | - | + | - | - |
| Arabinose | - | + | - | - | + |
| Xylose | + | + | + | + | - |
| Lactose | + | + | + | - | NA |
| Cellobiose | + | + | + | - | + |
| Sucrose | + | + | + | - | - |
| Maltose | + | + | + | - | - |
| Fucoidan | - | + | + | +/- | NA |
| ɩ-Carrageenan | + | - | + | +/- | NA |
| Xylan | + | - | - | NA | NA |
| Agar | + | - | - | - | - |
| Major nongaseous fermentation products* | Succinate, acetate, malate | Acetate, ethanol, lactate | Acetate, ethanol, lactate | Ethanol, acetate | Acid (maybe acetate**) |
| Nitrogen sources | N2, NH4+ | NH4+ | NH4+ | NH4+ | NH4+ |
a
Notations: NA, data not available. +, positive; -, negative; +/-, unstable, ceasing growth upon the second transfer. Data for strains other than NLcol2T were referenced from literatures: (a) (16); (b) (12); (c) (17). *Substrates were D-galactose for strain NLcol2T and D-glucose for other strains. **For strain S-5007T, acetate production was predicted from genomic data.