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. 2024 Jan 23;90(2):e01090-23. doi: 10.1128/aem.01090-23

Fig 2.

Fig 2

(A) Metabolic pathways of acetate oxidation and methanogenic pathways in the thermophilic acetate-fed chemostat, and (B) distribution of catabolic pathways among the studied contributors. For each syntroph and methanogen, we show the presence (indicated by filled circles) of genes encoding pathways for acetate catabolism and methanogenesis. Enzyme abbreviations are as follows: Ack, acetate kinase; Pta, phosphate acetyltransferase; Acs, acetyl-CoA synthetase; CODH complex, acetyl CoA synthetase complex; AcsE, methyltetrahydrofolate:corrinoid/iron-sulfur protein methyltransferase; MetF, methylenetetrahydrofolate reductase; FolD, methylenetetrahydrofolate dehydrogenase/cyclohydrolase; Fhs, formate-tetrahydrofolate ligase; Fdh, formate dehydrogenase; Grd, glycine reductase; Por, pyruvate dehydrogenase; PflD, pyruvate-formate lyase; Sda, serine dehydratase; GlyA, glycine hydroxymethyltransferase; GcvPA, glycine dehydrogenase subunit A; GcvPB, glycine dehydrogenase subunit B; GcvT, glycine cleavage system T protein; GcvH, glycine cleavage system H protein; Dld, dihydrolipoyl dehydrogenase. Fmd, formylmethanofuran dehydrogenase; Ftr, formylmethanofuran-tetrahydromethanopterin N-formyltransferase; Mch, methenyltetrahydromethanopterin cyclohydrolase; Mtd, methylenetetrahydromethanopterin dehydrogenase; Mer, F420-dependent 5,10-methenyltetrahydromethanopterin reductase; Cdh, acetyl-CoA decarbonylase/synthase complex; Mta, [methyl-Co(III) methanol-specific corrinoid protein]:CoM methyltransferase; Mtb, [methyl-Co(III) dimethylamine-specific corrinoid protein]:CoM methyltransferase; Mtm, [methyl-Co(III) monomethylamine-specific corrinoid protein]:CoM methyltransferase; Mtr, tetrahydromethanopterin S-methyltransferase; Mcr, methyl-CoM reductase. CHO-MF, formyl-methanofuran; CHO-H4MPT, formyl-tetrahydromethanopterin; CH≡H4MPT, methenyl-tetrahydromethanopterin; CH2=H4MPT, methylene-tetrahydromethanopterin; CH3-H4MPT, methyl-tetrahydromethanopterin; CH3-S-CoM, methyl-coenzyme M; HS-CoM, coenzyme M; HS-CoB, coenzyme B; CoB-S-S-CoM, mixed disulfide of CoM and CoB. Enzyme abbreviations and their corresponding genes are elaborated in Tables S3 and S5.