Table 1.
Enhanced induction of micronuclei in XRCC1-deficient cells
| Cell line | siRNA | Binucleated cells | Total micronuclei | |
|---|---|---|---|---|
| Scored | With micronuclei | |||
| MDA-MB-549 | XRCC1 | 800 | 243 | 444 |
| MDA-MB-549 | Scrambled | 800 | 190 | 262 |
| MDA-MB-549 | — | 800 | 174 | 245 |
| MDA-MB-453 | XRCC1 | 800 | 251 | 331 |
| MDA-MB-453 | Scrambled | 800 | 180 | 234 |
| MDA-MB-453 | — | 800 | 175 | 230 |
siRNA-transfected or untransfected MDA-MB-453 and MDA-MB-549 cells were treated with 100 or 150 μg/ml MMS, respectively, for 1 h, followed by an incubation in normal medium containing 3 μg/ml cytochalasin B for 48 h before analysis.