Table 1.
Strains and plasmids used in this study
| Genotype or relevant structures | Source or reference | |
|---|---|---|
| Strains | ||
| R1279 | CSH50 Δ(pho-bgl)201 Δ(lac-pro) ara thi | (16) |
| R2171 | As R1279 but attB::[tacOP-lacZ, bla]; RBS of phage T7gene10 in front of lacZ | pFDX3552→R1279 |
| Plasmids | ||
| M13mp18 | M13 phage cloning vector | (28) |
| pFDX500 | lacI, neo, ori p15A | (11) |
| pFDX3225 | φ(galK′-bglG) under control of Ptac, tet, ori p15A | (12) |
| pFDX3401 | λ int under control of λPR, λcI857 (cat; ori pSC101-repTS) | (12) |
| pFDX3551 | pLDR10 derivative for the insertion of a tacOP-lacZ cassette into the attB site; RBS of gene bglG in front of lacZ, bla, ori pMB1 | This study |
| pFDX3552 | As pFDX3551, but RBS of phage T7gene10 in front of lacZ | This study |
| pFDX4151 | As pFDX500, but with a NdeI site overlapping with the lacI ATG start codon | This study |
| pFDX4152 | As pFDX4151, but lacI fused to M13gene8 | This study |
| pFDX4153 | As pFDX4151, but lacI fused to λcI′ (codons 96–132) | This study |
| pFDX4154 | As pFDX4151, but lacI fused to galK′ (codons 1–32) | This study |
| pFDX4155 | As pFDX4151, but lacI fused to galK′-λcI′ | This study |
| pFDX4156 | As pFDX4151, but lacI fused to M13gene8-λcI′ | This study |
| pFDX4157 | As pFDX4152, but RBS of phage T7gene10 in front of φ(M13gene8-lacI) | This study |
| pFDX4158 | As pFDX4154, but RBS of phage T7gene10 in front of φ(galK′-lacI) | This study |
| pFDX4159 | As pFDX4155, but RBS of phage T7gene10 in front of φ(galK′-λcI′-lacI) | This study |
| pFDX4160 | As pFDX4156, but RBS of phage T7gene10 in front of φ(M13gene8-λcI′-lacI) | This study |
| pFDX4165 | As pFDX4151, but RBS of phage T7gene10 in front of lacI | This study |
| pLDR10 | Multiple cloning site, λattP, bla, cat, ori pMB1 | (14) |