FIG. 7.

A-CREB does not inhibit SRE-mediated gene expression. (A) A schematic representation of the pAF42SRE construct used in this experiment. (B) PC12 cells were transfected with the pAF42SRE reporter gene, an α-globin expression vector, and an empty vector or the A-CREB expression vector. Two days later, cells were treated with NGF (100 ng/ml, 45 min) or were left untreated and an RPA was performed. This experiment was performed three times with similar results.