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The AhR exists in a heterotetrameric complex in COS-1 cells. COS-1 cells were transfected with 9 μg of pcDNA3/βmAhR-FLAG or pcDNA3; cytosolic fractions were isolated, immunoabsorbed with M2 affinity gel, and resolved by SDS-PAGE; and the gel was silver stained. Lanes: 1, pcDNA3/βmAhR-FLAG; 2, pcDNA3/βmAhR-FLAG with M2 affinity gel blocked with FLAG peptide; 3, pcDNA3.
