FIG. 8.

XAP2 exists in a 0S to 4S complex in RL and shifts to 9S in the presence of mAhR. (A) pCI-XAP2 was transcribed and translated in rabbit RL with [³⁵S]methionine and resolved on a sucrose density gradient, and fractions were collected, resolved by SDS-PAGE, and analyzed by autoradiography. (B) XAP2 was cotranscribed and translated with mAhR in RL by the same procedure. (C) Cytosol isolated from COS-1 cells was resolved on a sucrose density gradient, and fractions were collected, resolved by SDS-PAGE, blotted, and probed with antibodies against hsp90 (3B6p90) or XAP2 followed by incubation with [¹²⁵I]GAM secondary antibody. (D to F) Quantitative assessments of panels A to C, respectively.