TABLE 2.

Temperature-induced expression of β-galactosidase from FKS2-lacZ reporter plasmids^a

FKS2-lacZ reporter plasmid	β-Galactosidase sp act (nmol/min/mg of protein)
	23°C	39°C	23°C + Ca2+
	3.3	67.2	51.8
	1.8	17.7	1.9
	1.8	17.1	2.0
	1.5	2.1	1.9

^aWild-type (1788) cells were transformed with the indicated reporter plasmids. Transformants were grown to saturation at 23°C in SD-Ura medium. These cultures were diluted into 20 ml of YEP containing 2% glucose (YEPD) so that subsequent incubation at either 23 or 39°C for 24 h resulted in mid-log-phase cultures (densities of 1.5 × 10⁷ to 3 × 10⁷ cells/ml). For the Ca²⁺ experiment, CaCl₂ was added to YEPD cultures to a final concentration of 30 mM, and the mixture was incubated for 24 h at 23°C. Crude extracts were prepared and enzyme activities were determined as described in Materials and Methods.