Fig. 3.

The effect of SD-MSs and RSD-MSs on BMSCs chondrogenic differentiation. (A) Alcian staining of the BMSCs cultured with SD-MSs and RSD-MSs (transwell) for 3, 7, and 14 days (scale bars 200 μm). (B) Quantification of Alcian blue staining. (C and D) Relative production of GAG and Col II for BMSCs cultured on SD-MSs and RSD-MSs at 3, 7, and 14 days. (E–I) In vitro expression of Col I, Col II, SOX9, ACAN, and Col X) in BMSCs cocultured with blank, SD-MSs and RSD-MSs for 7, and 14 days. (J) Immunofluorescence images of Col II, SOX9 and ACAN on BMSCs loaded on RSD-MSs after 14 days of induction (scale bars 100 μm). (K) Representative Western blot results of Col II, SOX9 and ACAN. (L) Quantitative analysis of Western blot.