Extended Data Fig. 8. Regulation of ligand-activated transcription factors by BBAAs in organoids.
RT-qPCR of target genes for FXR (Fgf15/FGF19), CAR3 (Cyp2b10/CYP2B6), PXR (Cyp3a11/CYP3A4), and AHR (Cyp1a1/CYP1A1) in (a) mouse and (b) human small intestinal organoids treated with Glu-CA or Glu-CDCA. Expression was calculated using the 2−ΔΔCt method, normalized to Gapdh/GAPDH. Error bars represent SD. (a) Biological replicates were n = 3 for vehicle and n = 4 for treatments (Cyp3a11 p = 4.8e-3**, p = 1.5e-2*, Cyp1a1 Glu-CA-50 p = 4.7e-4***, Glu-CA-100 p = 3.3e-4***, Glu-CDCA-100 p = 2.3e-3). (b) Biological replicates were n = 8 for vehicle and n = 6 for treatments across two independent experiments (p = 3.3e-3**). All significant differences between treatments and vehicle were determined using one-way ANOVA with Dunnett’s post-hoc test.