Table 4.

List of CellProfiler features used for profiling cellular morphology

Feature Group	Features and description
AreaShape	Compactness, Eccentricity, Extent, FormFactor, MajorAxisLength, MaxFeretDiameter, MaximumRadius, MeanRadius, MedianRadius, MinFeretDiameter, MinorAxisLength, Orientation, Perimeter, Solidity, Zernike shape features.
Correlation, Correlation_Manders, Correlation_RWC	Those correlations are calculated for pairs of channels.
Granularity	Granularity for each channel and each instance of the granularity spectrum.
Intensity	IntegratedIntensityEdge, LowerQuartileIntensity, MADIntensity, MassDisplacement, MaxIntensityEdge, MeanIntensity, MedianIntensity, MinIntensity, StdIntensityEdge, StdIntensity, UpperQuartileIntensity. Each feature is calculated per channel.
Location	CenterMassIntensity, MaxIntensity. Each location is calculated per channel.
Neighbors	AngleBetweenNeighbors, FirstClosestDistance, NumberOfNeighbors, PercentTouching, SecondClosestObjectNumber.Features in this group are only calculated for “Cell” and “Nuclei” groups.
RadialDistribution	FracAtD, MeanFrac, RadialCV. Each feature was calculated per channel.
Texture	AngularSecondMoment, Contrast, Correlation, DifferenceEntropy, DifferenceVariance, Entropy, Gabor, InfoMeas1\2, InverseDifferenceMoment, SumAverage, SumEntropy, SumVariance, Variance. Each feature was calculated per channel.