Fig. 2.

Analysis of FACS-purified BFCN. (A) Visualization of NGFR positive and negative septal cells after FACS. After immunostaining of dissociated cells from E18 mouse septa with anti-NGFR polyclonal antibody and Alexa-fluor conjugated secondary antibody, the cells were sorted and purified by FACS (see Materials and Methods), and aliquots of the positive and negative fractions were analyzed by phase and fluorescence microscopy. (B) RT-PCR of BFCN markers for Vacht (814 bp), Cht1 (840 bp), Chat (267 bp), Ngfr (657 bp), and Gapd (983 bp; as control) of RNAs obtained from NGFR positive and negative cell fractions.