Figure 4. Leukocyte distribution on model surface textures.

(A, B, C, D) Overlay of phase-contrast (blue) and fluorescent images (red indicating PBMCs) acquired 12 h after plating cells on different surfaces: flat (A), microtextured (B), and macrotextured ((C) for donor 1, (D) for donor 2). Scale bar, 200 μm. (E) Overlay of phase-contrast and fluorescent images (green for monocytes, red for lymphocytes) acquired at intervals of 0, 5, 10, and 15 h from the beginning of the experiment. Here, cells were plated on a macrotextured surface and co-cultured with S. epidermidis. Scale bar, 100 μm.