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(A) Upstream elements cooperate with ATF and MEF2 sites for EGF induction of the c-jun promoter. HeLa cells were transiently transfected with the indicated reporter plasmids (3 μg) and assayed for EGF induction of luciferase activity as described for Fig. 1. (B) Heterologous c-jun promoter constructs. The indicated fragments of the c-jun promoter were cloned upstream of a minimal c-fos promoter (shaded in grey) and a luciferase (LUC) reporter gene.
