FIG. 6.

Requirement of the MEF2 and ATF sites for Rac activation of the c-jun promoter. HeLa cells were transiently transfected with the indicated reporter genes, pCMV-β-galactosidase as an internal control, and either 2.5 μg of empty expression vector (pcDNA3) or racI(V12). After transfection, cells were serum starved in 0.2% newborn calf serum overnight and then lysed for luciferase and β-galactosidase assays. Relative luciferase activities normalized to the β-galactosidase activities are shown. The values shown are the averages of two separate experiments done in duplicate ± standard errors of the means.