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. 2024 Feb 21;15:1608. doi: 10.1038/s41467-024-45807-w

Fig. 5. Fine mapping of HPV capsid antigenicity.

Fig. 5

Mutational analysis of reference (lineage A) and outlier lineage for HPV33 (top panel; lineage B), HPV52 (middle panel; lineage D), and HPV58 (bottom panel; lineage C). Each panel comprises a cartoon depicting chimeric and mutant PsV sequences from reference lineage A and outlier lineage for each genotype. Heatmap presents natural log-transformed neutralizing antibody titers against each antigen using sera representing reference and outlier lineages. Capsomer crystal images representing HPV33 (PDB accession number: 6IGE.2), HPV52 (PDB accession number: 6IGF.1), and HPV58 (PDB accession number: 5Y9E.1) highlight variable residues between indicated lineages. Surface-exposed loops are highlighted as indicated in the key: BC (yellow), DE (pink), EF (red), FG (gold), and HI (green). Site polymorphisms were reported using standard nomenclature as X123Y wherein the first residue denotes the amino acid residue in the reference sequence, its position in that sequence, and then the residue in the outlier sequence at that same position. *, L2 fragment in clone AL1/BL2 differs from lineage B at two residues (V131 and N360), but shares three similar polymorphisms (N77, H350 and T372) and is otherwise identical to lineage B L2; L1 fragment in clone BL1/AL2 differs from lineage B by one residue (K495) but is otherwise identical to lineage B L1.