Table 2.
Key resources table.
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| Caspase 3: V450 Rabbit Anti-Active Caspase-3 (1/500) | BD Biosciences | Cat#560,627; RRID:AB_1727415; clone:C92-605 |
| PARP: BV421 Mouse Anti-Cleaved PARP (Asp 214) (1/500) | BD Biosciences | Cat#564,129; RRID:AB_2738611; clone:F21-852 |
| pSYK: Phospho-Syk (Tyr525/526) (C87C1) Rabbit mAb (PE Conjugate) (1/250) | Cell Signalling Technologies | Cat#6485; RRID:AB_11220429; clone:C87C1 |
| pPLCγ2: Alexa Fluor® 647 Mouse anti-PLC-γ2 (pY759) (1/100) | BD Biosciences | Cat#558,498; RRID:AB_647139; clone:K86-689.37 |
| pCD79a: Phospho-CD79A (Tyr182) (D1B9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (1/250) | Cell Signalling Technologies | Cat#52,821; RRID:AB_2799422; clone:D1B9 |
| Chemicals, peptides, and recombinant proteins | ||
| H2O2 | ThermoFisher | Cat#202,460,010 |
| RPMI Medium 1640 | Gibco | Cat#52,400–025 |
| FBS | Gibco | Cat#A3160801 |
| Penicillin/streptomycin | Gibco | Cat#15,140–122 |
| PFA | Merck | Cat#1,040,051,000 |
| Nuclease-free water | Ambion | Cat#AM9937 |
| Tris–HCl, pH 7.5 | Gibco | Cat#15,567–027 |
| NaCl | Sigma Aaldrich | Cat#S5886 |
| Triton X-100 | Thermo Fisher Scientific | Cat#85,112 |
| Dulbecco's phosphate buffered saline | Gibco | Cat#14,190–094 |
| 0.5 M EDTA, pH 8.0 | Lonza | Cat#51,201 |
| BSA | Sigma Aaldrich | Cat#A4503-50G |
| HFE-7500 3 M (TM) Novec (TM) Engineered fluid | Fluorochem | Cat#051,243 |
| Experimental models: Cell lines | ||
| Human: HBL1 | Gift from Prof. Dr. Annemiek van Spriel (Tumour Immunology, RadboudUMC) | RRID:CVCL_4213 |
| Software and algorithms | ||
| neMESYS UserInterface version 2016.6.14.1 | Cetoni | NA |
| BD FACSuite software v1.0.6 | BD Biosciences | NA |
| R version 4.0.5 | The R Foundation | https://www.r-project.org/ |
| RStudio | RStudio, USA | https://www.rstudio.com/ |
| BioRender | BioRender, Canada | https://biorender.com/ |
| Other | ||
| NeMESYS pump system | Cetoni | NA |
| Fine dosage syringe | Braun | Cat#9161406 V |
| HENKE-JECT hypodermic needle 0.6 × 25 mm | VWR | 613–2017 |
| PTFE tubing 0.81 mm DD × 1.63 mm OD | adtech | Cat#77,228 |