Figure 5.

Phagocytosis. Fluorescently labelled beads displaying Env-K biotinylated either with Bind&Bite (blue) or in an undirected (orange) manner were opsonized with saturating concentrations of the mAbs PG9 (A), PGT121 (B) or 5F3 (C) prior to incubation with THP-1 monocytic cells. Uptake of the beads by the THP-1 cells was determined by flow cytometry and the phagocytosis score was calculated as the percentage of bead-positive cells × MFI of bead-positive cells. Mean and standard deviations of triplicates from one representative of two independent experiments are shown. Non-opsonized beads were included as controls (dotted black line). Representative histogram of the flow cytometric analyses is shown for beads opsonized with the indicated antibodies at a concentration of 2.5 µg/ml. Statistical analyses were performed using a one-way ANOVA with Sidak’s multiple comparisons. *p<0.05.