Fig. 2.

Changes in intracellular pH affect the localization of TDP-43

A) Immunofluorescence of HEK293T cells overexpressing GFP-wtTDP-43-6*His, following a 1 h incubation at pH 7.2 or pH 6.4. White arrow: nuclear TDP-43; Yellow arrow: cytoplasmic TDP-43. Red arrow: TDP-43-positive puncta. Scale bar = 40 μm. B) Percentage of cells with TDP-43-positive puncta. *p = 0.04, Kruskal-Wallis test, N = 4. C) Localization of TDP-43 under different pH conditions. *p = 0.04, two-way ANOVA, N = 4. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)