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Ty1 IN localizes to the nucleus. Anti-IN antibody was used on cells induced for the production of E1-IN from the GAL1 promoter (A) or on cells induced for Ty1 transposition (B, panels K and L [panels M and N show GAL-LacZ negative controls]). FITC-stained panels are on the left; DAPI-stained panels are on the right. Constructs tested for IN localization are indicated to the left of the micrographs. FL, full length.
