TABLE 1.
Plasmids used in this study
| Plasmid | Markers | Comments | Source |
|---|---|---|---|
| pGEU414 | CEN TRP1 | GAL1 E1 | 28 |
| pGEU415 | CEN LEU2 | GAL1 E1 | 28 |
| pCAR224 | CEN LEU2 | C-terminal IN in p415GEU1 | This study |
| pCAR227 | CEN LEU2 | Full-length IN in p415GEU1 | This study |
| pCAR607 | CEN LEU2 | N-terminal IN in p415GEU1 | This study |
| pMAK60 | CEN TRP1 | Full-length IN in p414GEU1 | This study |
| pMAK61 | CEN TRP1 | INΔBglII-ClaI | This study |
| pMAK62 | CEN TRP1 | INΔEcoRI | This study |
| pMAK63 | CEN TRP1 | INΔClaI | This study |
| pMAK64 | CEN TRP1 | INΔClaI + EcoRI | This study |
| pMAK65 | CEN TRP1 | INΔBglII-ClaI/ΔEcoRI | This study |
| pMAK66 | CEN TRP1 | C-terminal IN in pGEU414 | This study |
| pMAK67 | CEN TRP1 | N-terminal IN in pGEU414 | This study |
| pX3 | CEN URA3 TRP1 | Ty1-TRP1, URA3 on plasmid | 58 |
| pMAK69 | CEN URA3 TRP1 | IN B1 in pX3 | This study |
| pMAK70 | CEN URA3 TRP1 | IN B2 in pX3 | This study |
| pGM315 | CEN URA3 TRP1 | Linker insertion mutation in IN | 51a |
| 90-1553 | CEN URA3 TRP1 | DD-YD change at YXDD domain in Ty1 RT | A. Gabriel (unpublished data) |
| pSD546 | 2 μm HIS3 bla | Target plasmid for in vitro IN assay | 26 |