Fig. 2. Comparison of the TMT and DIA based CETSA (tmtCETSA and diaCETSA). (A) The workflows of tmtCETSA and diaCETSA. (B) Bar charts showing the number of total proteins, kinases, significant kinases, and significant non-kinases by using tmtCETSA and diaCETSA. (C) Pearson correlation coefficient of protein intensities between two replicates by using tmtCETSA and diaCETSA. (D) Volcano plot visualization of kinase targets from K562 cell lysates, performed at 52 °C using 20 μM staurosporine by using tmtCETSA and diaCETSA. Adjusted p-value = 0.05 is indicated by a solid horizontal line. (E) Venn diagram displaying the kinase targets identified by classical TPP, tmtCETSA and diaCETSA methods. (F) Pie chart displaying the ratio of significant non-kinases that interact with kinases. (G) Interaction map of significant non-kinases PDCD10, PXN (tmtCETSA, right panel) and REHB (diaCETSA, left panel) interacting with kinases, respectively.