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Pdr13p does not act on the PDR1 promoter region. A wild-type strain was transformed with a low-copy-number plasmid containing a GAL1-PDR1 fusion gene. Along with this fusion gene, a high-copy-number plasmid containing the PDR13 gene (2μm PDR13) or the plasmid vector alone (vector) was introduced. Transformants containing each pair of plasmids were tested for their ability to grow on YPGE-galactose medium (YPGE-Gal) (49) lacking or containing cycloxheximide.
