TABLE 3.

Elevation of PDR5 expression by high-copy-number PDR13 requires the presence of PDR1^a

Fusion gene	β-Galactosidase activity (U/OD600) in the following strains in the presence of the indicated plasmid:
	Wild type		Δpdr1		Δpdr3		Δpdr1 pdr3
	Vector	2μm PDR13	Vector	2μm PDR13	Vector	2μm PDR13	Vector	2μm PDR13
PDR5-lacZ	39 ± 12	147 ± 33	22.5 ± 5	22.5 ± 6	44 ± 11	186 ± 45	2 ± 0.7	2 ± 0.5
TRP5-lacZ	29 ± 2	30 ± 4	31 ± 6	32.5 ± 6	35.5 ± 2	36 ± 10	41 ± 3	47 ± 8

^aA set of isogenic strains containing the indicated alleles of PDR1 and PDR3 were transformed with PDR5-lacZ and TRP5-lacZ fusion genes on low-copy-number plasmids. In addition, either the 2μm plasmid pRS424 (vector) or pRS424 carrying the wild-type PDR13 locus was introduced into each transformant. Transformants were grown and assayed for β-galactosidase activities as described in Table 2, footnote a.